| Place | Performance | Athlete | Team |
| 1 | 39.60 | Q: LAMPRECHT - DIVAN FRANCOIS | WESTERN CAPE MAINSTREAM |
| 2 | 40.46 | Q: BUTHELEZI - ANDILE | GAUTENG MAINSTREAM |
| 3 | 40.75 | q: VAN DER GUESSN - ANTON | GAUTENG MAINSTREAM |
| 4 | 41.31 | -: BOTES - KYLE | NORTH WEST MAINSTREAM |
| 5 | 41.50 | -: SIBANYONI - LWAZI | MPUMALANGA MAINSTREAM |