| Place | Performance | Athlete | Team |
| 1 | 39.62 | Q: HATTING - JUAN-FRANCO | GAUTENG MAINSTREAM |
| 2 | 40.40 | Q: MBATHA - NJABULO | KWAZULU NATAL MAINSTREAM |
| 3 | 41.42 | -: NIENABER - MARTIN JOHN | FREE STATE MAINSTREAM |
| 4 | 41.70 | -: NDLOVU - MTHO | MPUMALANGA MAINSTREAM |
| 5 | 42.89 | -: VAN GRAAN - GERHARD | EASTERN CAPE MAINSTREAM |
| 6 | 44.06 | -: TSHIVHANDEKANO - VHANGANI | LIMPOPO MAINSTREAM |