| Place | Performance | Athlete | Team |
| 1 | 44.04 | Q: WILSON - MIA | WESTERN CAPE MAINSTREAM |
| 2 | 44.80 | Q: VISSER - CARLI | NORTH WEST MAINSTREAM |
| 3 | 45.03 | Q: ENGELBRECHT - CHRISTINA ALIDA | WESTERN CAPE MAINSTREAM |
| 4 | 45.65 | -: LE ROUX - DINE | FREE STATE MAINSTREAM |
| 5 | 45.67 | -: VISSER - ALETHEA | GAUTENG MAINSTREAM |
| 6 | 46.14 | -: LOURENS - SIMON | MPUMALANGA MAINSTREAM |
| 7 | 47.18 | -: BUTZER - MADISON-LEE | GAUTENG MAINSTREAM |