| Place | Performance | Athlete | Team |
| 1 | 21.67 | Q: MARTIN - WIAAN | WESTERN CAPE MAINSTREAM |
| 2 | 21.95 | Q: RIKHOTSO - MBUYELO | MPUMALANGA MAINSTREAM |
| 3 | 22.37 | Q: MAKAMA - KGOTSO | GAUTENG MAINSTREAM |
| 4 | 22.46 | q: ZWANE - MOFIKI | FREE STATE MAINSTREAM |
| 5 | 22.71 | -: ENGELBRECHT - JAMEAL | NORTHERN CAPE MAINSTREAM |
| 6 | 23.01 | -: GUMEDE - MNQOBI | KWAZULU NATAL MAINSTREAM |