| Place | Performance | Athlete | Team |
| 1 | 21.68 | Q: SIBIYA - PHILABANDE | MPUMALANGA MAINSTREAM |
| 2 | 21.70 | Q: OLIPHANT - DAYLAN | NORTH WEST MAINSTREAM |
| 3 | 21.84 | Q: TITUS - DAIMAN | WESTERN CAPE MAINSTREAM |
| 4 | 22.18 | q: DLADLA - UNATHI | GAUTENG MAINSTREAM |
| 5 | 22.66 | q: MAXAMBELA - NTSIKA | EASTERN CAPE MAINSTREAM |
| 6 | 22.89 | -: VISAGIE - BRANDON | NORTHERN CAPE MAINSTREAM |