| Place | Performance | Athlete | Team |
| 1 | 22.04 | Q: LABUSCHAGNE - JANES | NORTHERN CAPE MAINSTREAM |
| 2 | 22.20 | Q: KIVIDO - JONATHAN | GAUTENG MAINSTREAM |
| 3 | 22.22 | Q: MASINGA - PROSPERITY | MPUMALANGA MAINSTREAM |
| 4 | 22.28 | q: DLADLA - BONGILE | NORTH WEST MAINSTREAM |
| 5 | 22.47 | q: STAFA - LUKHANYO DANZELL | FREE STATE MAINSTREAM |
| 6 | 22.57 | q: SAMPSON - RAYMONDRE QUEWIN | WESTERN CAPE MAINSTREAM |
| 7 | 22.60 | -: KATOO - BRYAN | EASTERN CAPE MAINSTREAM |