| Place | Performance | Athlete | Team |
| 1 | 22.57 | Q: MAKHAZA - SBONGAKONKE | KWAZULU NATAL MAINSTREAM |
| 2 | 22.89 | Q: SCHEEPERS - DYLAN | GAUTENG MAINSTREAM |
| 3 | 23.03 | Q: KERMIS - ELLIOT | WESTERN CAPE MAINSTREAM |
| 4 | 23.43 | q: OLIVIER - SAREL ALWYN | FREE STATE MAINSTREAM |
| 5 | 23.82 | q: LEBOTSA - TSHEPO | MPUMALANGA MAINSTREAM |