| Place | Performance | Athlete | Team |
| 1 | 22.55 | Q: MOULTRIE - COLE | EASTERN CAPE MAINSTREAM |
| 2 | 23.27 | Q: NGQWENISO - OWAMI | KWAZULU NATAL MAINSTREAM |
| 3 | 23.30 | Q: NIENABER - MARTIN JOHN | FREE STATE MAINSTREAM |
| 4 | 23.88 | -: NETSHITANGANI - UNARINE | LIMPOPO MAINSTREAM |
| 5 | 24.04 | -: CARROL - LEATHAN | NORTHERN CAPE MAINSTREAM |