| Place | Performance | Athlete | Team |
| 1 | 22.37 | Q: OLIVIER - ANDRE | NORTH WEST MAINSTREAM |
| 2 | 22.88 | Q: MAVANGU - DIVINE | GAUTENG MAINSTREAM |
| 3 | 23.12 | Q: MANCOTYWA - SIPHOSETHU | EASTERN CAPE MAINSTREAM |
| 4 | 23.15 | q: MLENGA - ANDILE | FREE STATE MAINSTREAM |
| 5 | 23.93 | -: MADONSELA - WEZILE | MPUMALANGA MAINSTREAM |
| 6 | 24.06 | -: DANNHAUSER - DANIEL | KWAZULU NATAL MAINSTREAM |
| 7 | 24.59 | -: MATAMBELE - JUNIOR | LIMPOPO MAINSTREAM |