| Place | Performance | Athlete | Team |
| 1 | 28.54 | Q: SIBIYA - NJABULO | MPUMALANGA LSEN |
| 2 | 28.99 | Q: SEABI - ZUKILE | NORTH WEST LSEN |
| 3 | 29.53 | Q: TSELA - MAHLOGONOLO | LIMPOPO LSEN |
| 4 | 32.10 | q: RIBA - MMAPHEFO | LIMPOPO LSEN |
| 5 | 34.24 | -: MOEKETSI - SIMON J | FREE STATE LSEN |
| 6 | 35.08 | -: BELANI - ALULUTHO | WESTERN CAPE LSEN |