| Place | Performance | Athlete | Team |
| 1 | 2:18.62 | Q: BRITZ - MIEKE LOUISE | FREE STATE MAINSTREAM |
| 2 | 2:18.63 | Q: PRETORIUS - HANRI | GAUTENG MAINSTREAM |
| 3 | 2:19.35 | Q: CHEMENE - MENTZ | GAUTENG MAINSTREAM |
| 4 | 2:19.37 | q: SITHOLE - SHEBBA | MPUMALANGA MAINSTREAM |
| 5 | 2:20.57 | q: VAN DER MERWE - SIMONE | WESTERN CAPE MAINSTREAM |
| 6 | 2:23.39 | q: VAN STADEN - INGE | FREE STATE MAINSTREAM |
| 7 | 2:28.75 | -: MSHIYO - ASEKHONA | EASTERN CAPE MAINSTREAM |
| 8 | 2:29.08 | -: MARITZ - DANE | NORTH WEST MAINSTREAM |
| 9 | 2:35.31 | -: STEYN - ANJA | KWAZULU NATAL MAINSTREAM |
| 10 | 2:40.01 | -: PHALANE - ROSINA | LIMPOPO MAINSTREAM |