| Place | Performance | Athlete | Team |
| 1 | 2:21.67 | Q: STEYNBERG - ANDREA NICOLA | FREE STATE MAINSTREAM |
| 2 | 2:21.68 | Q: LIGHTHELM - MIA | MPUMALANGA MAINSTREAM |
| 3 | 2:21.97 | Q: AUCAMP - MILA | WESTERN CAPE MAINSTREAM |
| 4 | 2:22.51 | q: DU PLESSIS - KATILYN | GAUTENG MAINSTREAM |
| 5 | 2:23.74 | q: LE ROUX - ELIZMA | GAUTENG MAINSTREAM |
| 6 | 2:27.16 | q: RAATGEVER - REECE | KWAZULU NATAL MAINSTREAM |
| 7 | 2:32.85 | -: SELEPE - MOLEBOHENG LAURAH | FREE STATE MAINSTREAM |
| 8 | 2:51.14 | -: KOLOTSI - NTHABISENG | LIMPOPO MAINSTREAM |
| 9 | 2:54.34 | -: NGOBENI - K | LIMPOPO MAINSTREAM |