| Place | Performance | Athlete | Team |
| 1 | 11.79 | Q: LE ROUX - KAYLEE | GAUTENG MAINSTREAM |
| 2 | 11.95 | Q: LA GRANGE - KAYLA | GAUTENG MAINSTREAM |
| 3 | 12.21 | Q: OOSTHUIZEN - SYDNEY | FREE STATE MAINSTREAM |
| 4 | 12.28 | q: BEUKES - ANIKA | WESTERN CAPE MAINSTREAM |
| 5 | 12.34 | q: SCHOONBEE - BIANCA | EASTERN CAPE MAINSTREAM |
| 6 | -: PRINSLOO - MEGAN | LIMPOPO MAINSTREAM | |
| 7 | -: PLAATJIES - MAXINE | NORTHERN CAPE MAINSTREAM |