| Place | Performance | Athlete | Team |
| 1 | 11.95 | Q: SWANEPOEL - ZANIQUE | GAUTENG MAINSTREAM |
| 2 | 12.19 | Q: ELLIOT - AMBER | EASTERN CAPE MAINSTREAM |
| 3 | 12.21 | Q: EZE - DIANA | GAUTENG MAINSTREAM |
| 4 | 12.28 | q: WANSBURY - CHARNE | FREE STATE MAINSTREAM |
| 5 | 12.36 | q: VAN NIEKERK - TENINKE | MPUMALANGA MAINSTREAM |
| 6 | 12.86 | -: GOVENDER - TIARA | KWAZULU NATAL MAINSTREAM |
| 7 | 13.89 | -: MORRIS - CYAL-LEE | NORTHERN CAPE MAINSTREAM |