| Place | Performance | Athlete | Team |
| 1 | 12.17 | Q: MAKGATO - MATSIDISO | MPUMALANGA MAINSTREAM |
| 2 | 12.24 | Q: VAN OUDTSHOORN - KAYLIE | GAUTENG MAINSTREAM |
| 3 | 12.48 | q: BOOYSEN - TAMRYN | WESTERN CAPE MAINSTREAM |
| 4 | 12.88 | -: CROUS - ANE | FREE STATE MAINSTREAM |
| 5 | 12.91 | -: STOFFBERG - KAYLIN | FREE STATE MAINSTREAM |
| 6 | 13.31 | -: NOVELA - MONICA | LIMPOPO MAINSTREAM |