| Place | Performance | Athlete | Team |
| 1 | 12.28 | Q: BANUTELO - BAGINYO KETSIA | FREE STATE MAINSTREAM |
| 2 | 12.41 | Q: FOUCHE - KEIRA | GAUTENG MAINSTREAM |
| 3 | 12.55 | q: CILLIERS - ZOë | MPUMALANGA MAINSTREAM |
| 4 | 12.55 | q: FICK - ELIZABETH | FREE STATE MAINSTREAM |
| 5 | 12.83 | -: LAMBRECHT - IZAAN | EASTERN CAPE MAINSTREAM |
| 6 | 12.97 | -: MAHLAPE - RELEBOGILE | LIMPOPO MAINSTREAM |