| Place | Performance | Athlete | Team |
| 1 | 14.01 | q: DE JAGER - ANGELIQUE | GAUTENG MAINSTREAM |
| 2 | 14.36 | q: SPERSHOT - GERMARIE | FREE STATE MAINSTREAM |
| 3 | 14.57 | q: VAN NIEKERK - TENINKE | MPUMALANGA MAINSTREAM |
| 4 | 14.64 | q: ABRAHAMS - KIERA LEE | WESTERN CAPE MAINSTREAM |
| 5 | 15.63 | -: NKOSI - TORISO | LIMPOPO MAINSTREAM |
| 6 | 16.34 | -: VAN NIEKERK - SIMONE | NORTH WEST MAINSTREAM |