| Place | Performance | Athlete | Team |
| 1 | 04:58.77 | Q: OELOFSE - DANELL | FREE STATE MAINSTREAM |
| 2 | 04:59.55 | Q: CONRADIE - ERNA-MARI | WESTERN CAPE MAINSTREAM |
| 3 | 04:59.82 | Q: ROBINSON - KIONA | GAUTENG MAINSTREAM |
| 4 | 05:00.01 | Q: VAN ROOYEN - KAITLYN | GAUTENG MAINSTREAM |
| 5 | 05:00.27 | q: LIEBENBERG - MATHILDA | NORTH WEST MAINSTREAM |
| 6 | 05:01.77 | q: BLIGNAUT - ANNARI | FREE STATE MAINSTREAM |
| 7 | 05:16.49 | q: ZULU - ANELE | KWAZULU NATAL MAINSTREAM |