| Place | Performance | Athlete | Team |
| 1 | 05:01.11 | Q: SCHWLST - RACHEL | GAUTENG MAINSTREAM |
| 2 | 05:01.27 | Q: DU PLESSIS - LU-MARI | MPUMALANGA MAINSTREAM |
| 3 | 05:05.00 | Q: HILLEBRAND - KARI SARAH | WESTERN CAPE MAINSTREAM |
| 4 | 05:10.82 | Q: STEYN - ANJA | KWAZULU NATAL MAINSTREAM |