| Place | Performance | Athlete | Team |
| 1 | 0:50.06 | Q: MOTIKO - MPHO | GAUTENG LSEN |
| 2 | 0:52.64 | Q: MHAMBI - BULELANI | NORTH WEST LSEN |
| 3 | 0:52.88 | Q: LOOTS - LIAN | WESTERN CAPE LSEN |
| 4 | 0:53.61 | q: SEHURUTSHE - KAMOGELO | FREE STATE LSEN |
| 5 | 0:56.48 | q: DREYER - BRADLEY | EASTERN CAPE LSEN |
| 6 | 0:59.16 | -: MNQAYI - MZOKHONA | KWAZULU NATAL LSEN |