| Place | Performance | Athlete | Team |
| 1 | 0:49.07 | Q: VAN RENSBURG - WERNICH | EASTERN CAPE MAINSTREAM |
| 2 | 0:49.15 | Q: MOLAETSA - MPHO | GAUTENG MAINSTREAM |
| 3 | 0:49.17 | Q: SPANGENBERG - DIVAN | GAUTENG MAINSTREAM |
| 4 | 0:50.03 | q: JACOBS - SLADE | WESTERN CAPE MAINSTREAM |
| 5 | 0:51.91 | -: MEIRING - GINO | NORTHERN CAPE MAINSTREAM |
| 6 | 0:52.00 | -: MORERWA - KGOTHATSO | LIMPOPO MAINSTREAM |