| Place | Performance | Athlete | Team |
| 1 | 0:56.91 | Q: NGONGO - AKONYI | GAUTENG MAINSTREAM |
| 2 | 0:57.87 | Q: NOTNAGEL - LINAMARE | GAUTENG MAINSTREAM |
| 3 | 0:58.98 | Q: SAAIMAN - HAILEY | MPUMALANGA MAINSTREAM |
| 4 | 1:00.70 | -: JULIE - NOMHLE | FREE STATE MAINSTREAM |
| 5 | 1:02.39 | -: SCHMIDT - KLAUDIA | WESTERN CAPE MAINSTREAM |