| Place | Performance | Athlete | Team |
| 1 | 0:56.86 | Q: JONKER - ALETTA | GAUTENG MAINSTREAM |
| 2 | 0:58.24 | Q: KOTZE - JUANNE | FREE STATE MAINSTREAM |
| 3 | 0:59.20 | Q: HONNIBAL - DANIELLE | EASTERN CAPE MAINSTREAM |
| 4 | 0:59.47 | q: VAN ZYL - LUANDI | LIMPOPO MAINSTREAM |
| 5 | 1:00.00 | q: VAN WYK - ZANDR | MPUMALANGA MAINSTREAM |