| Place | Performance | Athlete | Team |
| 1 | 0:48.06 | Q: THOMAS - HILTON | GAUTENG MAINSTREAM |
| 2 | 0:48.85 | Q: LABUSCHAGNE - JANES | NORTHERN CAPE MAINSTREAM |
| 3 | 0:49.16 | q: PIENAAR - JEAN JACQUES WOUTER | FREE STATE MAINSTREAM |
| 4 | 0:49.59 | q: ESSIEN - JONATHAN AYEYI | WESTERN CAPE MAINSTREAM |
| 5 | 0:49.74 | -: GELDENHUYS - WIHAN | MPUMALANGA MAINSTREAM |
| 6 | 0:50.04 | -: PIETERSE - JUSTIN | GAUTENG MAINSTREAM |
| 7 | 0:51.11 | -: MOKGAWA - SELAELO | LIMPOPO MAINSTREAM |