| Place | Performance | Athlete | Team |
| 1 | 0:49.08 | Q: V.D.LITH - ZANDER | GAUTENG MAINSTREAM |
| 2 | 0:49.24 | Q: RAMMUDU - RORISANG | LIMPOPO MAINSTREAM |
| 3 | 0:49.72 | -: BOSCH - DAMIAN | WESTERN CAPE MAINSTREAM |
| 4 | 0:51.16 | -: MAGANGA - KHAYALENKOSI | NORTHERN CAPE MAINSTREAM |
| 5 | 0:51.29 | -: NCWANE - SQINISEKO | KWAZULU NATAL MAINSTREAM |