| Place | Performance | Athlete | Team |
| 1 | 0:49.94 | Q: FRAZER - WJ | MPUMALANGA MAINSTREAM |
| 2 | 0:50.45 | Q: NHLAPO - NKOSINATHI | GAUTENG MAINSTREAM |
| 3 | 0:50.57 | -: TAYENZA - JARAVADZA | KWAZULU NATAL MAINSTREAM |
| 4 | 0:51.39 | -: CROUCAMP - BRAAM | NORTH WEST MAINSTREAM |
| 5 | 0:52.39 | -: MATHIPA - KHOLOFELO | LIMPOPO MAINSTREAM |