| Place | Performance | Athlete | Team |
| 1 | 0:56.76 | Q: THOMPSON - MICHAELA | NORTH WEST MAINSTREAM |
| 2 | 0:57.24 | Q: ACKERMAN - ELIZABETH PETRONELLA C | FREE STATE MAINSTREAM |
| 3 | 0:58.61 | -: LAUBSCHER - CHARLIZE | GAUTENG MAINSTREAM |
| 4 | 1:00.68 | -: TERBLANCHE - TIA | KWAZULU NATAL MAINSTREAM |
| 5 | 1:00.77 | -: NDLOVU - TSAKANE | LIMPOPO MAINSTREAM |