| Place | Performance | Athlete | Team |
| 1 | 0:57.14 | Q: DE BEER - MIA | NORTH WEST MAINSTREAM |
| 2 | 0:57.85 | Q: GRIESSEL - ZOE | EASTERN CAPE MAINSTREAM |
| 3 | 0:58.24 | q: VILJOEN - JANKE - MARTINE | WESTERN CAPE MAINSTREAM |
| 4 | 0:59.08 | -: RADEBE - PABALLO | FREE STATE MAINSTREAM |
| 5 | 0:59.40 | -: MULDER - JANE | GAUTENG MAINSTREAM |
| 6 | 0:59.51 | -: PRINSLOO - CHANTE | GAUTENG MAINSTREAM |