| Place | Performance | Athlete | Team |
| 1 | 0:51.16 | Q: KOEKEMOER - LEENDERT | GAUTENG MAINSTREAM |
| 2 | 0:53.15 | Q: BADLI - ZUKO | EASTERN CAPE MAINSTREAM |
| 3 | 0:53.22 | -: BUSH - JO-WAINE JAYDEN | WESTERN CAPE MAINSTREAM |
| 4 | 0:53.75 | -: BUYS - TIAAN | NORTH WEST MAINSTREAM |
| 5 | 0:53.86 | -: OLIVIER - SAREL ALWYN | FREE STATE MAINSTREAM |
| 6 | 0:55.53 | -: RESHAYUM - DONE | KWAZULU NATAL MAINSTREAM |