| Place | Performance | Athlete | Team |
| 1 | 0:52.79 | Q: PIENAAR - ALEX | GAUTENG MAINSTREAM |
| 2 | 0:53.38 | Q: OCKS - JADON | WESTERN CAPE MAINSTREAM |
| 3 | 0:53.87 | -: DE NECKER - ADRIAN | FREE STATE MAINSTREAM |
| 4 | 0:54.94 | -: KANYANE - AMOANDRIES | LIMPOPO MAINSTREAM |
| 5 | 0:55.28 | -: MEHLO - BULELANI | NORTHERN CAPE MAINSTREAM |