| Place | Performance | Athlete | Team |
| 1 | 0:50.93 | Q: ROMIJN - KRYN | MPUMALANGA MAINSTREAM |
| 2 | 0:51.65 | Q: JORDAAN - JOSHUA | EASTERN CAPE MAINSTREAM |
| 3 | 0:51.69 | q: CHIZOMBO - HAPPY | GAUTENG MAINSTREAM |
| 4 | 0:52.78 | q: ENSLIN - JOHAN | WESTERN CAPE MAINSTREAM |
| 5 | 0:53.70 | -: DA SILVA - DIEGO | GAUTENG MAINSTREAM |
| 6 | 0:54.59 | -: NIEUWOUDT - BJORN | FREE STATE MAINSTREAM |