| Place | Performance | Athlete | Team |
| 1 | 0:58.18 | Q: PRETORIUS - HANRI | GAUTENG MAINSTREAM |
| 2 | 0:59.15 | Q: BOTES - ANZELLE | WESTERN CAPE MAINSTREAM |
| 3 | 1:00.58 | -: KOTZE - AMY | FREE STATE MAINSTREAM |
| 4 | 1:01.42 | -: LOURENS - SIMON | MPUMALANGA MAINSTREAM |
| 5 | 1:02.47 | -: MADLAKUVANA - SISIPHO | EASTERN CAPE MAINSTREAM |
| 6 | 1:03.22 | -: VISSER - VISSER JANIE | NORTHERN CAPE MAINSTREAM |