| Place | Performance | Athlete | Team |
| 1 | 0:57.85 | Q: DE VILLIERS - JOHANKA | GAUTENG MAINSTREAM |
| 2 | 0:57.87 | Q: VAN HEERDEN - KEIRA | EASTERN CAPE MAINSTREAM |
| 3 | 0:58.05 | q: VISSER - CARLI | NORTH WEST MAINSTREAM |
| 4 | 0:59.22 | q: VERSTER - NECKE | FREE STATE MAINSTREAM |
| 5 | 0:59.76 | -: LIGHTHELM - MIA | MPUMALANGA MAINSTREAM |
| 6 | 1:00.86 | -: VAN STADEN - INGE | FREE STATE MAINSTREAM |
| 7 | 1:01.75 | -: RAATGEVER - REECE | KWAZULU NATAL MAINSTREAM |