| Place | Performance | Athlete | Team |
| 1 | 26.41 | -: HAASBROEK - TSIDKE | WATERKLOOF DOGTERS A |
| 2 | 27.14 | -: OKOYE - ALEXANDRA | NWU INVITATIONAL TEAM |
| 3 | 27.94 | -: VAN NIEKERK - XANE | WATERKLOOF DOGTERS B |
| 4 | 28.03 | -: BOSHOFF - ANUSHKA | EG JANSEN |
| 5 | 28.96 | -: SELOTA - VELAMINA | VORENTOE GIRLS |
| 6 | 29.03 | -: VAN DER WESTHUIZEN - ELZAAN | MIDSTREAM IND |
| 7 | 29.04 | -: NEL - ZURITA | EG JANSEN |