| Place | Performance | Athlete | Team |
| 1 | 44.86 | VISSER - CARLI | WESVALIA DOGTERS |
| 2 | 45.95 | PIENAAR - DANYELLE | ORANJE MEISIES |
| 3 | 46.52 | NIEMAND - MEGAN | MENLOPARK DOGTERS A |
| 4 | 46.56 | JENKINS - CAYLEN | NOORDHEUWEL DOGTERS |
| 5 | 47.41 | BOUWER - KARLA | GARSFONTEIN DOGTERS |
| 6 | 47.43 | DE VILLIERS - LINE | MENLOPARK DOGTERS A |
| 7 | 47.59 | ACHURCH - KAYLIN | AFFIES MEISIES BLOU |
| 8 | 48.08 | GUNTER - ISABELLA | MONTANA GIRLS |
| 9 | 48.70 | JV RENSBURG - MILLA | AFFIES MEISIES BLOU |
| 10 | 48.73 | DITTMER - INKE | WATERKLOOF DOGTERS A |
| 11 | 48.96 | LOMBARD - KAYLI | MENLOPARK DOGTERS B |
| 12 | 49.02 | VAN DER WALT - MIEKE | NWU INVITATIONAL TEAM |
| 13 | 49.04 | LOURENS - SIMON | NELSPRUIT |
| 14 | 49.35 | HAMILTON - MEAGAN | EG JANSEN |
| 15 | 49.79 | DU PLESSIS - MARLENE | ORANJE MEISIES |
| 16 | 49.84 | COLLET - JASMINE | CURRO HAZELDEAN WHITE GIRLS |
| 17 | 49.86 | BISSCHOFF - KAYLEE | MONTANA INV |
| 18 | 49.99 | HUDSON - KAYLA | SECUNDA GIRLS INV |
| 19 | 50.01 | SWART - RIEMKE | NWU INVITATIONAL TEAM |
| 20 | 50.04 | SCHOLTZ - MINETTE | GARSFONTEIN DOGTERS |
| 21 | 50.39 | BELLINGAN - ALLISON | HELPMEKAAR WIT DOGTERS |
| 22 | 50.50 | OOSTHUIZEN - MIA | MARAIS VILJOEN DOGTERS |
| 23 | 50.60 | SEPUPANE - BOKAMOSO | CURRO HAZELDEAN WHITE GIRLS |
| 24 | 50.68 | VD MERWE - CHARLIZE | ELDORAIGNE DOGTERS |
| 25 | 51.03 | VAN SCHALKWYK - LEANDRI | WESVALIA DOGTERS |
| 26 | 51.15 | OBERHOLSTER - HANNAH | DINAMIKA |
| 27 | 52.02 | VAN DER MERWE - AME | WATERKLOOF INV |
| 28 | 52.30 | OELOFSE - MIEKE | WATERKLOOF INV |
| 29 | 52.31 | VAN DER WAL - CHENE | RUSTENBURG DOGTERS |
| 30 | 52.37 | PIENAAR - MCKENNA | ORANJE MEISIES UIT |
| 31 | 52.41 | AVIS - KENDRA | LEEUWENHOF |
| 32 | 52.58 | DU PLESSIS - ELRIKE | BRITS GIRLS |
| 33 | 52.69 | WILLIAMS - TENIQUE | EG JANSEN |
| 34 | 52.85 | PRYOR - CORNELLE | JEUGLAND |
| 35 | 53.00 | LOTZ - ELIZCA | ERMELO GIRLS |
| 36 | 53.01 | PRETORIUS - ANRI | MIDSTREAM GIRLS |
| 37 | 53.09 | LE ROUX - ANE | RANDBURG DOGTERS |
| 38 | 53.62 | STOKES - ZANDRI | OOS-MOOT DOGTERS |
| 39 | 53.88 | CASTELYN - ANKE | MONTANA GIRLS |
| 40 | 54.16 | TANNOUS - AVA | KLERKSDORP GEEL DOGTERS |
| 41 | 54.44 | VAN VUUREN - AMELIA | HANS MOORE DOGTERS |
| 42 | 54.45 | DE KOOKER - KAYDEN | HELPMEKAAR GOUD DOGTERS |
| 43 | 54.62 | O'GRADY - VENNIQUE | VEREENIGING GIM DOGTER |
| 44 | 55.84 | LABUSCHAGNE - JONNIKE | DINAMIKA |
| 45 | 55.96 | SWART - QUINET | NWU INVITATIONAL TEAM |
| 46 | 56.16 | GROBLER - LEILA | MIDSTREAM GIRLS |
| 47 | 58.01 | VENTER - TATUM | DR MALAN DOGTERS |
| 48 | 58.63 | JAGER - MIA | MONUMENT |
| 49 | 59.56 | RAATH - ILA | NWU INVITATIONAL TEAM |
| 50 | 60.29 | MASILO - SHANDY | VEREENIGING GIM DOGTER |