| Place | Performance | Athlete | Team |
| 1 | 0:46.68 | Q: NENE - ZAKITHI | KWAZULU NATAL ATHLETICS |
| 2 | 0:46.91 | Q: ISAACS - GARDEO | BOLAND ATHLETICS |
| 3 | 0:47.37 | Q: MOHOLOBELA - KABELO | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:47.47 | q: MAVUNDLA - OSCAR | ATHLETICS GAUTENG NORTH |
| 5 | 0:47.55 | -: VAN DER WALT - TJAART | ATHLETICS FREE STATE |
| 6 | 0:48.04 | -: SEELIGER - JAMES | ATHLETICS GAUTENG NORTH |
| 7 | 0:48.22 | -: VAN DER MERWE - KEEGAN | WESTERN PROVINCE ATHLETICS |
| 8 | 0:48.60 | -: LOTTERING - DARYLL | EASTERN PROVINCE ATHLETICS |