| Place | Performance | Athlete | Team |
| 1 | 23.97 | Q: SHABANGU - BANELE | ATHLETICS GAUTENG NORTH |
| 2 | 25.53 | Q: STOLZ - JENNA | CENTRAL GAUTENG ATHLETICS |
| 3 | 25.95 | -: KING - GERALDINE | WESTERN PROVINCE ATHLETICS |
| 4 | 26.14 | -: ENGLER - ZOE | KWAZULU NATAL ATHLETICS |
| 5 | 27.25 | -: BALOYI - FANISA | LIMPOPO ATHLETICS |
| 6 | -: VAN DER MERWE - NICOLA | ATHLETICS CENTRAL NORTHWEST | |
| 7 | -: MOLOI - REABETSWE | ATHLETICS GAUTENG NORTH | |
| 8 | -: FOX - CHRISTIN | ATHLETICS CENTRAL NORTHWEST |