| Place | Performance | Athlete | Team |
| 1 | 23.23 | Q: NEKHUBUI - SHIRLEY | EASTERN PROVINCE ATHLETICS |
| 2 | 23.27 | Q: THOMAS - TAMZIN | ATHLETICS CENTRAL NORTHWEST |
| 3 | 23.87 | q: SEKESE - MPHONTSHENG | ATHLETICS GAUTENG NORTH |
| 4 | 24.76 | q: MOGWASI - SOKWAKANA | ATHLETICS FREE STATE |
| 5 | 24.94 | -: NGEMA - SNETHEMBA | KWAZULU NATAL ATHLETICS |
| 6 | 25.01 | -: LEFIFI - WANITA | ATHLETICS GAUTENG NORTH |
| 7 | 25.22 | -: DE JAGER - RENCIA | ATHLETIS SOUTH WESTERN DISTRICTS |
| 8 | 28.25 | -: VAN DER HEEVER - DESMORENE | NORTHWEST CAPE ATHLETICS |