| Place | Performance | Athlete | Team |
| 1 | 23.58 | Q: COETZEE - MIRANDA | ATHLETICS NORTWEST NORTH |
| 2 | 24.74 | Q: LE ROUX - KAYLEE | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.80 | -: LOTTER - ZAINE | BOLAND ATHLETICS |
| 4 | 25.19 | -: ARENDSE - SHANNON | WESTERN PROVINCE ATHLETICS |
| 5 | 25.35 | -: BEUKES - ANIKA | ATHLETIS SOUTH WESTERN DISTRICTS |
| 6 | 28.36 | -: MHLOMI - NOLITHA | ATHLETICS TRANSKEI |
| 7 | -: NEL - RHOD | ATHLETICS MPUMALANGA | |
| 8 | -: ROTHMAN - AMIEL | ATHLETICS CENTRAL NORTHWEST |