| Place | Performance | Athlete | Team |
| 1 | 25.34 | ESTERHUYSE - VEN | HTS MIDDELBURG A |
| 2 | 25.41 | VAN OUDTSHOORD - KAYLIE | NOORDHEUWEL A |
| 3 | 25.49 | WILSON - MIA | LA ROCHELLE |
| 4 | 25.51 | VAN DER MERWE - ZANTE | AFFIES MEISIES A |
| 5 | 25.93 | ROCHA - SELENA | TUKS SPORT SCHOOL A |
| 6 | 26.22 | BOTES - ZENIQUE | GARSFONTEIN A |
| 7 | 26.33 | VERMAAK - HANNAH HOPE | NOORDHEUWEL A |
| 8 | 26.43 | MAKASI - LOLWETHU | TUKS INVITATIONAL |
| 9 | 26.48 | WILSON - CARA | LA ROCHELLE |
| 10 | 26.52 | NIEMANDT - KARA | MENLOPARK B |
| 10 | 26.52 | FOURIE - MILA | TUKS INVITATIONAL |
| 12 | 26.68 | MARX - MIELET | AFFIES MEISIES B |
| 13 | 26.88 | KINNEAR - HAYLEIGH | MARAIS VILJOEN A |
| 14 | 26.99 | DE BEER - ARETHA | OOS-MOOT |
| 15 | 27.01 | VENTER - ZELDIE | DR MALAN B |
| 16 | 27.11 | LE ROUX - ALICIA | HELPMEKAAR BRUIN |
| 17 | 27.17 | OPPERMAN - KEIRA -LEE | TUKS INVITATIONAL |
| 18 | 27.31 | BOSHOFF - ANUSHKA | EG JANSEN |
| 19 | 27.39 | VERSTER - NECKE | ORANJE MEISIES A |
| 20 | 27.41 | STOFFBERG - KAYLIN | WITTEBERG DOGTERS |
| 21 | 27.85 | LOUW - MARIAAN | ORANJE MEISIES A |
| 22 | 27.96 | VAN DER WESTHUIZEN - ELZAAN | MIDSTREAM WHITE |
| 23 | 28.12 | OPPERMAN - NADIA | TUKS INVITATIONAL |
| 24 | 28.33 | WOLFAARDT - JAIMEE | CORNWALL HILL A |
| 25 | 28.49 | MERAFE - RORISANG | TUKS INVITATIONAL |
| 26 | 28.63 | VAN NIEKERK - XAN | TUKS INVITATIONAL |
| 27 | 28.80 | MCPHERSON - AMY | TUKS INVITATIONAL |
| 28 | 29.03 | BOTHA - ALEXA | CURRO HAZELDEAN |
| 29 | 29.37 | LOUW - ELANI | TUKS INVITATIONAL |
| 30 | 29.76 | CURRY - CAITLIN | BENONI GIRLS |
| 31 | 29.99 | VAN STADEN - RUNE | HARTBEESPOORT |
| 32 | 30.12 | LABUSCHAGNE - LIZENE (F) | DIE WILGERS |
| 33 | 30.67 | RILEY - JASMINE | PRETORIA NOORD B |
| 34 | 31.87 | ROUX - TOANETTE | WONDERBOOM B |