| Place | Performance | Athlete | Team |
| 1 | 25.96 | DE KLERK - AMAE | HELPMEKAAR WIT |
| 2 | 26.27 | BURGER - RUME | ORANJE MEISIES A |
| 3 | 26.31 | FICK - ELIZABETH | TUKS INVITATIONAL |
| 4 | 26.54 | BOOYSEN - TAMERON | LA ROCHELLE |
| 5 | 26.60 | RADEMEYER - LERUSHKE | AFFIES MEISIES D |
| 6 | 26.76 | MEYER - NICOLE | MONTANA B |
| 7 | 26.82 | DU PLOOY - LUANNE | MARAIS VILJOEN A |
| 8 | 26.91 | EKSTEEN - SUMARIE | WITTEBERG DOGTERS |
| 9 | 27.20 | TIVANE - SONIA | SECUNDA GIRLS |
| 10 | 27.29 | UNGERER - LANA | EG JANSEN |
| 10 | 27.29 | DE BEER - IZEL | TUKS INVITATIONAL |
| 12 | 27.55 | CHOENE - XYLIA | ORANJE MEISIES A |
| 13 | 27.61 | OTTE - EMMA | MIDSTREAM TEAL |
| 14 | 27.70 | VAN DER MERWE - PAULA | WAGPOS |
| 15 | 27.80 | ALBERTS - BROOKLYN | BENONI GIRLS |
| 16 | 27.98 | HEUNIS - LILANY | OOS-MOOT |
| 17 | 28.06 | DE WAAL - MIA | MENLOPARK C |
| 18 | 28.69 | CLASEN - JADE | BRANDWAG |
| 19 | 29.01 | BURGER - JEANDRI | TUKS INVITATIONAL |
| 20 | 29.65 | KIDD - HAYLEY | CURRO WINDHOEK GYMNASIUM B |
| 21 | 29.88 | VAN DEVENTER - LARISSA | WAGPOS |
| 22 | 30.21 | OOSTHUIZEN - MIA | MARAIS VILJOEN B |
| 23 | 30.36 | MOKOENA - MBALI | JOHAN JURGENS |
| 24 | 30.46 | HERBST - NINKE (F) | DIE WILGERS |
| 25 | 31.08 | VAN DYK - ALIDA | MARAIS VILJOEN B |
| 26 | 33.41 | MAODI - PRINCESS | AMANDASIG |