| Place | Performance | Athlete | Team |
| 1 | 48.76 | -: VERHOEF - NATALIE | OVERKRUIN |
| 2 | 48.99 | -: VAN DER WALT - MIGHAN | MIDSTREAM MAROON |
| 3 | 49.00 | -: VAN WYK - SOPHIA | MERENSKY |
| 4 | 49.23 | -: VAN JAARSVELD - SHANIQUE | GARSFONTEIN B |
| 5 | 49.64 | -: MAKINK - CHANTEL | AFFIES MEISIES D |
| 6 | 50.57 | -: JONKER - MINANTE | HARTBEESPOORT |
| 7 | 54.41 | -: MULLER - DANIQUE | MENLOPARK DOGTERS C |