| Place | Performance | Athlete | Team |
| 1 | 0:41.36 | RELAY TEAM - | GAUTENG MAINSTREAM |
| 2 | 0:42.35 | RELAY TEAM - | MPUMALANGA MAINSTREAM |
| 3 | 0:43.18 | RELAY TEAM - | WESTERN CAPE MAINSTREAM |
| 4 | 0:43.56 | RELAY TEAM - | LIMPOPO MAINSTREAM |
| 5 | 0:45.10 | RELAY TEAM - | FREE STATE MAINSTREAM |
| 6 | 0:46.59 | RELAY TEAM - | NORTHERN CAPE MAINSTREAM |