| Place | Performance | Athlete | Team |
| 1 | 25.06 | Q: LATEGAN - MEGAN | CENTRAL GAUTENG ATHLETICS |
| 2 | 25.37 | Q: ESTERHUYSE - VEN | ATHLETICS MPUMALANGA |
| 3 | 25.72 | Q: BOTES - ZENIQUE | ATHLETICS GAUTENG NORTH |
| 4 | 25.82 | q: EDROSS - JALEELA | WESTERN PROVINCE ATHLETICS |
| 5 | 26.34 | -: BOOYSEN - TAMRYN | BOLAND ATHLETICS |
| 6 | 26.66 | -: ROCHA - SELENA | ATHLETICS GAUTENG NORTH |
| 7 | 26.77 | -: FOURIE - ASTRID | ATHLETICS VAAL TRIANGLE |
| 8 | 27.20 | -: VENTER - ZELDIE | ATHLETICS VAAL TRIANGLE |