| Place | Performance | Athlete | Team |
| 1 | 0:53.00 | Q: VAN RENSBURG - WERNICH | EASTERN PROVINCE ATHLETICS |
| 2 | 0:53.18 | Q: VAN DER MERWE - HANDRE | ATHLETICS MPUMALANGA |
| 3 | 0:53.23 | Q: RAMATHE - KGOMTSO | ATHLETICS VAAL TRIANGLE |
| 4 | 0:53.30 | q: DE BEER - MICHAEL | LIMPOPO ATHLETICS |
| 5 | 0:59.01 | -: FRIEDBURG - BENO-WADE | NORTH WEST CAPE ATHLETICS |
| 6 | 1:01.06 | -: SIBIYA - PHILASANDE | ATHLETICS MPUMALANGA |