| Place | Performance | Athlete | Team |
| 1 | 25.63 | Q: VENTER - ANEBEL | ATHLETICS FREESTATE |
| 2 | 25.92 | Q: VAN DER MERWE - ZANTE | ATHLETICS GAUTENG NORTH |
| 3 | 26.12 | Q: ROCHA - SELENA | ATHLETICS GAUTENG NORTH |
| 4 | 26.19 | q: HAASBROEK - TSIDKE | ATHLETICS GAUTENG NORTH |
| 5 | 26.22 | q: BOOYSEN - TAMRYN | BOLAND ATHLETICS |
| 6 | 26.82 | -: BOTES - ANZELLE | ATHLETICS SOUTH WESTERN DISTRICT |