| Lane | Number | Athlete | Team |
| 1 | 25 | SWANEPOEL LELANIE | CENTRAL GAUTENG ATHLETICS |
| 2 | 7 | KILLIAN ELIANA | ATHLETICS GAUTENG NORTH |
| 3 | 16 | OOSTHUIZEN MARY-AMY | ATHLETICS VAAL TRIANGLE |
| 4 | 1 | MARX LEE-ANN | ATHLETICS CENTRAL NORTHWEST |
| 5 | 28 | LOUW CARA | LIMPOPO ATHLETICS |
| 6 | 24 | HENDRIKS SHADE | CENTRAL GAUTENG ATHLETICS |
| 7 | 26 | DU TOIT LIANDIE | KWAZULU NATAL ATHLETICS |
| 8 | 3 | LOUW ROLINE | ATHLETICS FREE STATE |