| Place | Performance | Athlete | Team |
| 1 | 0:41.82 | RELAY TEAM - | ATHLETICS GAUTENG NORTH |
| 2 | 0:42.02 | RELAY TEAM - | WESTERN PROVINCE ATHLETICS |
| 3 | 0:42.06 | RELAY TEAM - | ATHLETICS MPUMALANGA |
| 4 | 0:43.22 | RELAY TEAM - | BOLAND ATHLETICS |
| 5 | 0:44.10 | RELAY TEAM - | ATHLETICS FREE STATE |
| 6 | 0:44.14 | RELAY TEAM - | ATHLETICS VAAL TRIANGLE |
| 7 | 0:44.67 | RELAY TEAM - | CENTRAL GAUTENG ATHLETICS |