| Place | Performance | Athlete | Team |
| 1 | 0:45.93 | Q: MAVUNDLA - OSCAR | MASAI |
| 2 | 0:46.50 | Q: SEMENYA - JOEL | MASAI |
| 3 | 0:46.58 | -: SHEZI - TUMISANG | TUT |
| 4 | 0:48.56 | -: PHASHA - THATO | MASAI |
| 5 | 0:49.40 | -: MASHIGO - KARABO | MASAI |
| 6 | 0:49.64 | -: PHEKO - KARABELO | TUKS |
| 7 | 0:52.01 | -: MADZIDZELA - NTSIKELELO | TUKS |