| Place | Performance | Athlete | Team |
| 1 | 26.02 | Q: HAASBROEK - TSIDKE | WATERKLOOF |
| 2 | 26.42 | Q: VAN ROOYEN - KATHLEEN ISABELLA | MENLOPARK |
| 3 | 27.23 | Q: VERHOEF - NATALIE | OVERKRUIN |
| 4 | 27.27 | -: NGUBINE - SINDISIWE | MAMELODI |
| 5 | 29.38 | -: NKOSI - TAWONGA | JS |
| 6 | 30.53 | -: MYNHARDT - LA-ROCHELLE | ORYX |